Development of the Unani Anti-HIV Drug HOO-IMM PLUS

The formulation of the drug is made according to the Unani system of Medicine and approved by FDA (Food and Drug Administration), Mumbai, India. And the compositions of the formulation are purely herbal, standardized according to the Pharmacopoeia of Herbal Extracts.

The R&D of this particular drug initially started as a treatment for the asymptomatic and symptomatic HIV associated diseases and AIDS related complex. And later with the considerable improvement in the patients noted in response to the therapy more scientific and controlled studies were undertaken with the help of modern molecular diagnostic services from reputed and a well-equipped laboratory 'Reliance Life Science, Siro-Prolego, Nicholas Piramal Lab etc.

The formulation has undergone scientific studies for the two most important tools for analyzing the effectiveness of the drug; they are a) Invitro Studies and b) Invivo Studies. Also the formulation has successfully gone through a toxicity test and certified for free from heavy metals, steroid and animal origins, lead, arsenic, mercury etc. The medicines are prepared according to FDA GPM Standard specifications, following the traditional time proven Indigenous Indian ethics of Unani- Ayurvedic system in concurrence with the most modern medical findings, exercising our own innovative techniques for developing drugs for the diseases like HIV, Leukemia, Hepatitis & Liver disorders, Heart Diseases & Diabetes related complication etc. confronting the cotemporary world.

The medicines fulfill the most stringent quality demands of the statutory requirements as well as the International Quality Management.

Composition of the Unani Anti-HIV drugs HOO-IMM PLUS: - HOO-IMM PLUS (A)

1. Saffron (Zafran) Pg292* - Restorative, builds up body's defense mechanism thereby strengthening the immunity system considerably.
2. Orchis Latifolia (Salab Misri) Pg147* - Tonics for blood, vital energy and vital essence.
3. Pipper Longum (Phil Phil Daras) Pg 119* - Strengthens up spleen, lymphatic glands. Activates the endocrine glands of the body.
4. Peganum Harmala (Hurmul) Pg 61* Increases level of cellular immunity; inhibits replication of viral activities.
5. Pipper Nigrum (Kali Miri) Pg 336* - A Pulmonary tonic, important in long term strengthening of the lungs.
6. Withania Somnifera (Asgandh) Pg 64* - A Nervine tonic, helps relieve associated psychological tension or anxiety.
7. Sida Cordifolia (Beej Band) Pg 140* - A Diuretic, relieves the congestive nature accompanied by water retention.
8. Pearl (Moti) Pg345* - Increases amount of calcium, iodine and phosphorus in the body.

HOO-IMM PLUS (B)

1. Pistachia lentiscum (Masthagi) Pg339* - Tonics for blood, vital energy and vital essence.
2. Hollerrhena Antidysenterica (Inderjo Talkh) Pg79* - Anti-diarrheal relieving dysenteric symptoms.
3. Emblica Officinalis (Amla) Pg 49* - Strengthens up lymphatic glands by promoting lymphocyte transformation.
4. Arista Lochia (spp) (Zaravant) Pg 140* - An Anti-microbial, helps the body control and clear bacterial infection.
5. Zingiber Officinalis (Zanjabeel) Pg 55* - An Expectorant remedy, helps to maintain a minimum build up of sputum in the lungs.
6. Pistachia Vera Linn (Gul Pista) Pg122* - Enhance renal functions.
7. Punica Granatum Linn (Gul Anar) Pg75* - Prevents degeneration of renal tubes & nephrons.

HOO-IMM PLUS (C)

1. Santalum Album (Sandal) Pg 166* - Enhances new cells regeneration, cleans up dead cells & prevents pus formation.
2. Similex China Linn (Chob Chini) Pg167* - Accelerates protein synthesis, thus stimulating cell regeneration.
3. Piper Cubeba (Kabab Chini) Pg 263* - Accelerates protein synthesis, thus stimulating cell regeneration.
4. Swertia Charata (Chiraita Shirin) Pg160*- Detoxify the impure blood.
5. Borage Officinalis (Gav Zuban) Pg296* -Increases level of cellular immunity; inhibits growth of tumor cells.
6. Centaurea Behen (Behman Surkh) Pg108* - Anti-neoplastic, prevents development & maturation of malignant cells.
7. Lavendula Steochas (Istukhudus) Pg63*- Strengthens up spleen, lymphatic and endocrine glands.
HOO-IMM PLUS (D)

1. Delphinium Denudatum (Jadhwar) Pg 359*- An Anti-microbial, helps the body control and clear bacterial infection.
2. Planta Goovata (Isabgol) Pg62* - Promote bowel movement through stimulation of bile production in the liver.
3. Chena Podium Album (Bathwa) Pg 93* - Circulatory Stimulant, promotes the circulation of blood and thus oxygen availability for vascular resistance,
4. Artemisia Absinthium (Afzantheen) Pg 66*- A Vascular Tonic, helps nourish the tissue of the arteries and veins. Prevents against hardening of fibrous muscles of the Heart.
5. Arista Lochia (spp) (Zaravant) Pg 207* - An An Anti-spasmodic, eases the muscle spasms, cramps that are the immediate cause of pain.
6. Terminalia Arjun (Arjun) Pg 57* - Cardiac & Vascular Tonic, supports the tissue of the cardiovascular system, maintaining flexibility and tones affected vessels.
7. Cinnamomum Zeyanicum (Dalchini) Pg181* - This drug rectifies all mucus membrane in the body after reducing the virus.

HOO-IMM PLUS (E).

1. Swertia Charata (Chiraita Shirin) Pg160*- Detoxify the impure blood. Antipyretic subsides persisting fever
2. Kawal Gatta (Nelumbu Nucifera) Pg283* - Relieves congested & catarrhal conditions of liver and controls secretion of bile.
3. Meba Azadrach (Bakain) Pg 100* - Anti-tumor immuno-surveillant system, intensifies phagocytosis of reticulo-endothelial systems.
4. Rubia Cordifolia (Majeeth) Pg 335*- Accelerates protein synthesis, thus stimulating cell regeneration.
5. Valerina Officinalis (Balched) Pg 91* - Hepato-protective, detoxifying the liver with the deleterious effects of daily encounters with air-water and food-borne toxins.
6. Chena Podium Album (Bathwa) Pg 93* - Circulatory Stimulant, promotes the circulation of blood and thus oxygen availability for vascular resistance,
7. Berberis Vulgaris (Dar Hald) Pg 182* - Anti-microbial, facilitates the body ridding itself of pathogens present reducing inflammation and its resulting symptoms

Invitro Lab Studies
In invitro lab studies, this drug was put for Drug Sensitivity Test and Anti-retroviral Activity Test in the laboratory adhering to the standard protocol and the highest inhibition rate of 98% was recorded against the causative agent, Human Immunodeficiency Virus.

Invivo Clinical Studies
In the Invivo human clinical studies, these drugs were provided as a treatment to the HIV patients through HAKH Medical Foundation and the improvements in the patients physically as well as pathologically were recorded in proper, controlled conditions through standard procedure and all the blood test pertaining to HIV were undertaken through Molecular Diagnostic Services. The clinical observation of this drug revealed the following improvements in the patients: -
The immediate responses of HOO-IMM PLUS Treatment on HIV patient are:-

• Curtails and relieves the initial symptoms of HIV such as fever, diarrhea, fatigueness , skin diseases etc. within a month
• Increment in body weight of 1.5 - 3 kg a month
• 25-35% augments inCD4count in first month and doubles within 4 months.
• 7-8 folds decrease in HIV viral load counts within 3 months.
• Restores to the healthy condition enabling to lead a productive life.

The gradual responses of HOO-IMM PLUS Treatment are:-

• Viral load count decreased to less than detectable limit (20 copies) 7, 8
• Tones up functions of vital organs of the body.
• Reduce & stop the vulnerability to opportunistic infections.
• Immunity, vigor & vitality of the patient are restored enabling him to lead a productive energetic life.
• Acts as a prophylactic to prevent transmission of HIV from mother to fetus during pregnancy.
• Eliminates the genetic Gag region of HIV namely P55, P39, P17 & Pol integrase P51.
• Eliminates the HIV Antigen from the body proven by DNA PCR Antigen Test.

Safety studies of the treatment are:-

• The Toxicological Studies, Storage Stability Studies and are being successfully made from the FDA Approved Lab.
• Purely herbal ingredients confirmed in HPTLC
• These medicines proved to be free from any inclusion of Steroids or Heavy Metals.
• These medicines are free from all toxic or spurious ingredients therefore granted Free Sale Certificate by FDA.
• No side-effects and drug interactions were noted in the patients.
• The patient Liver Function Test after treatment observed to be normal.

Drug Resistance to the treatment:-

• Throughout our study for more than 10 years now, we have never come across patients with drug resistance towards HOO-IMM PLUS Treatment.
• And the same treatment continued for the whole course without any resistance in respect rebounding of viral load count etc.
• No interference noted in terms of side-effects or contra-indications when taken along with ART in the patients those were undergoing ART and started HOO-IMMPLUS.

Phenomenal change in DNA-PCR Antigen Test
Patients those continued the treatment for 18-24 months, once attaining the viral load to less than detectable limit and adhering to the instruction strictly, had recorded HIV DNA-PCR Qualitative Antigen Test 'Not Detected' and the test interpreted the blood sample to be negative for the presence of HIV. And so the medications were completely stopped for almost one year and again undergone for HIV DNA-PCR Antigen test (Qualitative) and again recorded 'Not Detected'. The same test was repeated for 2nd, 3rd & 4th year after completely stopping the medications and the patients yet recorded 'Not Detected' for the test.
The method for the confirming the presence of HIV antigen is HIV DNA-PCR Antigen test (Qualitative) test by Nested PCR Technique. _{2, 3}

A major breakthrough in HIV Treatment: (Past HIV Genetic Structure Vs Present Structure)
A major finding was uncovered when the patient's earlier Western blot antibody test before starting the treatment was compared with the present western blot test after the treatment. The HIV-1 intermediate Gag precursors P55, P39 and matrix protein P17 and Pol integrase P51 were found to be absent in all the patients who had recorded 'Not Detected' in an HIV DNA-PCR antigen test after the treatment.

The matrix protein P17 MA also plays an important role in the HIV-1 viral life cycle. P17 is responsible for the transfer of viral nucleo capsids from the nuclei to plasma membranes, the site of virus assembly. Thus Gag precursors P39 & Matrix Protein P17 are responsible for a redirection of the assembly process to the membrane of the endoplasmic reticulum.

Thus indicating the absence in the process of new viral assembly of HIV in the body.

Western Blot Kit Used: Genelab HIV Blot 2.2 (US FDA Approved) 5, 6

An Edge over the other Anti-HIV drug.
No consideration over the following issues:-

1. When to start the therapy.
2. Consideration for pregnant women.
3. Affect on liver for co-infected hepatitis patients.
4. Side-effects & drugs interactions.
5. Multi drug resistance and STIs.
6. High cost for different combination.

Felicitation & Appreciation:-

• Indian Parliament debate on 25th Aug 2004. (Lok Sabha question No. 4112).
• All India Hakeem Ajmal Khan Award being conferred for "Invention of Unani Formula for HIV/AIDS " by Union Health Minister Dr. Anbumani Ramadoss on 28th Sept 2005.
• Global Award 2006 for "The only Anti-HIV Drug, proven by DNA-PCR Antigen Test" by Health Minister, New Delhi, Dr. Yoganand Shastri on 27th Dec 2006.
• Global Award 2006 for "Inventor of HIV/AIDS Medicines" by Health Minister New Delhi, Dr. Yoganand Shastri on 27th Dec 2006.
• 23rd International European Convention Award for Innovation & Technology in Medical Science on 19 Feb 2007.
• Ibnu Sina National Award 2007 for "Invention of HIV/AIDS Medicine in Tibb-e-Unani" by HoD of Central Council for Unani Research & Medicine (CCRUM), India held in Mumbai on 18th March 2007.
• Best Innovative Unani Drug Manufacturing Award 2011 by Dr. Salman Khurshid, Union Cabinet Minister, Law and Justice, India held in New Delhi on 10th March 2011 on World Unani Day.
• International Excellence Award 2012 by Eng. Redha Hassan Salman, Director of Public Health & Safety Department, Dubai for the Best Research & Development of Innovative Unani Drugs in a conference held in Burj- Al- Arab, Dubai on 11th June 2012.

Regarding any symptoms & doctors Consultation & advice: - We have panels of doctors specialized in Herbal, Ayurvedic, Unani system of medicine especially for HIV/AIDS. Therefore any need for consultation regarding the disease, symptoms, treatment etc you can always contact us through email or online consultation.

E- Treatment: - The patients can now avail the treatment with HOOIMM PLUS from any remote areas throughout the world with the help of Online Registration & Consultation. The patients have to just register themselves with our website www.hootone.org can avail online consultation whereby they will be provided treatment through courier services and free consultation services. For all your queries, online doctor's consultation available at info@hootone.org
*Reference:-

1. From Kithabul Mufarrada- FDA recognized Referential book for Unani Medicines.
2. Molecular Virology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research.
3. Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, Pennsylvania.
4. Journal of Clinical Microbiology. 2004 June; 42(6): 2742-2751.
5. WHO Collaborating Group on HIV- 2 1990, WHO Weekly Epidem. Rec. 10, p74- 75.
6. CDC. 1985. United States Morbidity and Mortality Weekly Report 34 (1): 1- 5
7. Palmer S., Wiegand A.,Coffin J et al., 2003. New Real-Time Reverse Transcriptase PCR Assay with Single-Copy Sensitivity for Human Immunodeficiency Virus Type-1 RNA in Plasma. J Clinical Microbiol., 41, 4531-4536.
8. Saag MS, Holodniy M, Kuritzkes DR, et al. HIV viral load markers in clinical practice. Nat. Med. 1996; 2:625-9.

The cost of the treatment for 2 months is mentioned below:-

SR.No.	MEDICINE	QTY	RATE	AMOUNT
1	HOOIMM-PLUS (A, B, C, D, E)	2	$ 100	$ 200
2	BALANTC	2	$ 15	Complimentary
3	HOOCLEANSE	2	$ 10	Complimentary
4	COURIER CHARGE			$60
	TOTAL			$ 260

The cost of the treatment for 4 months is mentioned below:-

SR.No.	MEDICINE	QTY	RATE	AMOUNT
1	HOOIMM-PLUS (A, B, C, D, E)	4	$ 100	$ 200
2	BALANTC	4	$ 15	Complimentary
3	HOOCLEANSE	4	$ 10	Complimentary
4	COURIER CHARGE			$60
	TOTAL			$ 460

Payment can be made in any of the following ways: -

1. FOR ONLINE PAYMENT:-
   Steps to be followed:-
   You can log on to our website:-
   1. http://hootone.org/online-purchase.html
   2. http://latesthivtreatment.org/online-purchase.html

And Click on the top side righten side "Online Purchase"

• New page opens
• Enter the Quantity you intend to purchase in the box under "Quantity" and then click update.
• Then click "Proceed to checkout" Button placed on the right side bottom.
• New page opens.
• Click on the top middle button tilting downwards to see the details of purchase
• If the details of purchase match with your requirement then fill the form beneath
• And then click the bottom button" Review Order and Continue"

2 WESTERN UNION MONEY TRANSFER
For the Receiver's Name and address kindly log on to

1. http://hootone.org/wumgram.html
2. http://latesthivtreatment.org/wumgram.html

3 BANK REMITTANCE OR BANK SWIFT
You can also opt for direct bank deposit to our company Bank account. For bank account details log to:-

1. http://hootone.org/wrtransfer.html
2. http://latesthivtreatment.org/wrtransfer.html

Once the payment is made, you have to intimate us the payment details along with your complete address. And your medicines will be dispatched through DHL and the tracking number be provided to you on the very next day. And the medicines will be delivered within 4-5 working days.

Please feel free to contact us for any information required.
Anticipating from you,
Yours Truly,
Dr. Sayyed Habeeb
Medical Officer
HOOTONE REMEDIES
00-91-9594000093